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Yeast and bacteria growth has been detected by observing light diffracted from specially prepared diffracting
screens upon which these organisms were grown. The screens were fabricated by impressing patterns on the
surface of a growing medium. As the microbes metabolized, they caused severe changes in the lightdiffracting
properties of the patterns as they ate into them. Such changes were detected within twenty
minutes for bacteria and ten minutes for yeast.
Ramendra Deo Bahuguna,Gareth T. Williams,Iraj K. Pour, andR. Raman
"Study of microbial growth II: by holographic interferomery", Proc. SPIE 1332, Optical Testing and Metrology III: Recent Advances in Industrial Optical Inspection, (1 January 1991); https://doi.org/10.1117/12.51060
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Ramendra Deo Bahuguna, Gareth T. Williams, Iraj K. Pour, R. Raman, "Study of microbial growth II: by holographic interferomery," Proc. SPIE 1332, Optical Testing and Metrology III: Recent Advances in Industrial Optical Inspection, (1 January 1991); https://doi.org/10.1117/12.51060