Sensitive fiber-optic immunoassay

Irene M. Walczak; Walter F. Love; Rudolf E. Slovacek

doi:10.1117/12.43859

1 July 1991 Sensitive fiber-optic immunoassay

Irene M. Walczak, Walter F. Love, Rudolf E. Slovacek

Proceedings Volume 1420, Optical Fibers in Medicine VI; (1991) https://doi.org/10.1117/12.43859
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The principles of evanescent wave theory were applied to an immunological sensor for detecting the cardiac-specific isoenzyme creatine kinase-MB (CK-MB). The detection of the CK-MB isoenzyme is used in conjunction with the total CK measurement in the diagnosis of acute myocardial infarction. The clinical range for CK-MB is from 2-100 ng/ml. Previous work which utilized the fluorophor, Fluorescein isothiocyanate (FITC), was able to discriminate between 0 and 3 ng/ml CK-MB. Use of the fluorophor B-phycoerythrin (BPE) increased the assay sensitivity to 0.1 ng/ml CK-MB. The data was collected for 15 minutes using an optical launch and collection angle of 25 degree(s). This fiber optic based system is homogeneous and requires no subsequent washing, handling, or processing steps after exposure to the sample.

Citation Download Citation

Irene M. Walczak, Walter F. Love, and Rudolf E. Slovacek "Sensitive fiber-optic immunoassay", Proc. SPIE 1420, Optical Fibers in Medicine VI, (1 July 1991); https://doi.org/10.1117/12.43859

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