Photon correlation spectroscopic studies of filamentous actin networks

Jay Edward Newman; Pier L. San Biagio; Kenneth L. Schick

doi:10.1117/12.44159

1 June 1991 Photon correlation spectroscopic studies of filamentous actin networks

Jay Edward Newman, Pier L. San Biagio, Kenneth L. Schick

Proceedings Volume 1430, Photon Correlation Spectroscopy: Multicomponent Systems; (1991) https://doi.org/10.1117/12.44159
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

Actin plays a major role in the structural integrity and motility of cells as well as in the intracellular dynamics of other macromolecules. Photon Correlation Spectroscopy (PCS) has been used to monitor the diffusion of polystyrene latex spheres (PLS) of different sizes within in vitro polymerized actin solutions under a variety of conditions. Specific actin-binding proteins were added to regulate the actin filament lengths as well as to cross-link filaments together. PCS measurements give information on the mobility of PLS over probing distances equal to the inverse scattering vector magnitude which range from 40 to 420 nm for the data. Results allow estimation of the mean pore sizes within the actin networks as a function of both actin concentration (0.4 - 5 mg/ml) and the presence of actin-binding proteins. Probe diffusion coefficients were measured for PLS samples in length-regulated actin networks at a fixed actin concentration, c (0.65 mg/ml) as c*, the semi-dilute overlap concentration, was varied. There was a change in the dependence on c/c* near the value 1 at which the filaments just overlap. Quasi-static low-angle diffraction patterns from actin solutions have been detected and quantified using a CCD video camera/frame grabber system to study the spatial and slow temporal variations in the intensity patterns.

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Jay Edward Newman, Pier L. San Biagio, and Kenneth L. Schick "Photon correlation spectroscopic studies of filamentous actin networks", Proc. SPIE 1430, Photon Correlation Spectroscopy: Multicomponent Systems, (1 June 1991); https://doi.org/10.1117/12.44159

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