Background correction in multiharmonic Fourier transform fluorescence lifetime spectroscopy

Kerry M. Swift; George Weston Mitchell

doi:10.1117/12.44188

1 May 1991 Background correction in multiharmonic Fourier transform fluorescence lifetime spectroscopy

Kerry M. Swift, George Weston Mitchell

Proceedings Volume 1431, Time-Resolved Spectroscopy and Imaging of Tissues; (1991) https://doi.org/10.1117/12.44188
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

We report here a method for measuring and eliminating artifacts caused by background in fluorescence lifetime or rotational correlation data using multiharmonic Fourier transform frequency domain fluorometry. A single measurement on a blank yields phase and amplitude values for multiple modulation frequencies simultaneously, and these may then be used to correct the sample's data. Experimental results demonstrate the applicability of the method over a wide range of background contributions.

Citation Download Citation

Kerry M. Swift and George Weston Mitchell "Background correction in multiharmonic Fourier transform fluorescence lifetime spectroscopy", Proc. SPIE 1431, Time-Resolved Spectroscopy and Imaging of Tissues, (1 May 1991); https://doi.org/10.1117/12.44188

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