Analysis of neuropeptides using capillary zone electrophoresis with multichannel fluorescence detection

Jonathan V. Sweedler; Jason B. Shear; Harvey A. Fishman; Richard N. Zare; Richard H. Scheller

doi:10.1117/12.50463

1 December 1991 Analysis of neuropeptides using capillary zone electrophoresis with multichannel fluorescence detection

Jonathan V. Sweedler, Jason B. Shear, Harvey A. Fishman, Richard N. Zare, Richard H. Scheller

Proceedings Volume 1439, International Conference on Scientific Optical Imaging; (1991) https://doi.org/10.1117/12.50463
Event: International Conference on Scientific Optical Imaging, 1990, Georgetown, Cayman Islands

Abstract

Capillary zone electrophoresis is fast becoming one of the most sensitive separation schemes for sampling complex microenvironments. A unique detection scheme is developed in which a charge-coupled device (CCD) detects laser induced fluorescence from an axially illuminated electrophoresis capillary. The fluorescence from an analyte band is measured over a several centimeter section of the capillary, greatly increasing the observation time of the fluorescently tagged band. The sensitivity of the system is in the 1-8 X 10-20 mol range for derivatized amino acids and peptides. Subattomole quantities of bag cell neuropeptides collected from the giant marine mollusk Aplysia californica can be measured.

Citation Download Citation

Jonathan V. Sweedler, Jason B. Shear, Harvey A. Fishman, Richard N. Zare, and Richard H. Scheller "Analysis of neuropeptides using capillary zone electrophoresis with multichannel fluorescence detection", Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); https://doi.org/10.1117/12.50463

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