Scanning force microscopy of cells and membrane proteins

David Keller; Leda Chang; Ke Luo; Seema Singh; Maxim Yorgancioglu

doi:10.1117/12.58194

1 May 1992 Scanning force microscopy of cells and membrane proteins

David Keller, Leda Chang, Ke Luo, Seema Singh, Maxim Yorgancioglu

Proceedings Volume 1639, Scanning Probe Microscopies; (1992) https://doi.org/10.1117/12.58194
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Recent results on scanning force microscopy of cells and membrane proteins are presented. Whole immune system cells (rat basophil leukemia cells) can be imaged either alive and moving in aqueous medium, frozen, and exposed by freeze fracture (and imaged at -25 degree(s)C), fixed with glutaraldehyde in buffer, or fixed and dried (as if for scanning electron microscopy). Living cells can be stimulated with antigens or drugs and then observed as they move and change shape as a function of time after exposure. In either living or fixed cells it is possible to visualize and map cytoskeletal networks under the cell membrane, and, in living cells, to observe changes in the network with time. Membrane proteins (e.g., the F1 fragment of ATP synthase) can be imaged by simple passive adsorption to freshly cleaved mica. The resolution is about 50 angstroms, which is high enough to identify individual protein molecules, but still too low to distinguish internal structure. Factors which limit resolution and methods that may overcome these limitations are also discussed.

Citation Download Citation

David Keller, Leda Chang, Ke Luo, Seema Singh, and Maxim Yorgancioglu "Scanning force microscopy of cells and membrane proteins", Proc. SPIE 1639, Scanning Probe Microscopies, (1 May 1992); https://doi.org/10.1117/12.58194

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