Photoinduced electron transfer and fluorescence mechanisms in covalently linked polynuclear aromatic-nucleotide complexes

Nicholas E. Geacintov; Bing Mao; Luisa L. France; Rushen Zhao; Junxin Chen; Tong Ming Liu; Nai-Qi Ya; Leonid A. Margulis; John Clark Sutherland

doi:10.1117/12.58273

1 April 1992 Photoinduced electron transfer and fluorescence mechanisms in covalently linked polynuclear aromatic-nucleotide complexes

Nicholas E. Geacintov, Bing Mao, Luisa L. France, Rushen Zhao, Junxin Chen, Tong Ming Liu, Nai-Qi Ya, Leonid A. Margulis, John Clark Sutherland

Author Affiliations +

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58273
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

The fluorescence of polycyclic aromatic hydrocarbon-nucleic acid complexes is quenched by photoinduced electron transfer mechanisms in aqueous solutions at ambient temperatures. These effects are illustrated with the biologically important compound benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), a mutagenic and carcinogenic metabolite of the environmental pollutant benzo[a]pyrene, which forms covalent mutagenic lesions with 2'-deoxyguanosine (dG) residues in DNA. The dependence of the fluorescence yield and fluorescence decay times of the covalent model adduct (+)-trans- BPDE-N²-dG as a function of temperature and methanol/water composition are described. Because of the sensitivity of the fluorescence of the pyrenyl residue to the polarity of the microenvironment, the magnitude of the fluorescence yield can be used to distinguish between highly hydrophobic (e.g., intercalation) and other more solvent-exposed BPDE- nucleic acid binding sites.

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Nicholas E. Geacintov, Bing Mao, Luisa L. France, Rushen Zhao, Junxin Chen, Tong Ming Liu, Nai-Qi Ya, Leonid A. Margulis, and John Clark Sutherland "Photoinduced electron transfer and fluorescence mechanisms in covalently linked polynuclear aromatic-nucleotide complexes", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58273

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