Pathological considerations of laser-tissue interactions: light microscopic assessment of thermal damage of skin

Thomas J. Flotte M.D.; Margaret Goetschkes

doi:10.1117/12.137355

1 June 1992 Pathological considerations of laser-tissue interactions: light microscopic assessment of thermal damage of skin

Thomas J. Flotte M.D., Margaret Goetschkes

Author Affiliations +

Proceedings Volume 1643, Laser Surgery: Advanced Characterization, Therapeutics, and Systems III; (1992) https://doi.org/10.1117/12.137355
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64 - 66 degree(s)C, 80 - 85 degree(s)C, and > 100 degree(s)C. The best choices based on this study include the following: fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: the modified elastic stain presented in the appendix of this paper.

Citation Download Citation

Thomas J. Flotte M.D. and Margaret Goetschkes "Pathological considerations of laser-tissue interactions: light microscopic assessment of thermal damage of skin", Proc. SPIE 1643, Laser Surgery: Advanced Characterization, Therapeutics, and Systems III, (1 June 1992); https://doi.org/10.1117/12.137355

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