Antibody-based bacterial toxin detection

Darrell E. Menking; Jonathon M. Heitz; Nabil A. Anis; Roy G. Thompson

doi:10.1117/12.170668

10 March 1994 Antibody-based bacterial toxin detection

Darrell E. Menking, Jonathon M. Heitz, Nabil A. Anis, Roy G. Thompson

Proceedings Volume 2068, Chemical, Biochemical, and Environmental Fiber Sensors V; (1994) https://doi.org/10.1117/12.170668
Event: Optical Tools for Manufacturing and Advanced Automation, 1993, Boston, MA, United States

Abstract

Fiber optic evanescent fluorosensors are under investigation in our laboratory for the study of drug-receptor interactions for detection of threat agents and antibody-antigen interactions for detection of biological toxins. In a one step assay, antibodies against Cholera toxin or Staphylococcus Enterotoxin B were noncovalently immobilized on quartz fibers and probed with fluorescein-isothiocyanate (FITC)-labeled toxins. In the two-step assay, Cholera toxin or Botulinum toxoid A was immobilized onto the fiber, followed by incubation in an antiserum or partially purified antitoxin IgG. These were then probed with FITC-anti-IgG antibodies. Unlabeled toxins competed with labeled toxins or antitoxin IgG in a dose-dependent manner and the detection of the toxins was in the nanomolar range.

Citation Download Citation

Darrell E. Menking, Jonathon M. Heitz, Nabil A. Anis, and Roy G. Thompson "Antibody-based bacterial toxin detection", Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); https://doi.org/10.1117/12.170668

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