Laser-based blood cell measurements for the biological reconstruction of ionizing radiation doses using a novel technique for cytometry/biochemical reaction analysis

Pavel G. Pleshanov; Richard G. Langlois; L. A, Azarov; D. A. Budayev; A. L. Ossipenkov; D. M. Shamayev

doi:10.1117/12.180807

21 July 1994 Laser-based blood cell measurements for the biological reconstruction of ionizing radiation doses using a novel technique for cytometry/biochemical reaction analysis

Pavel G. Pleshanov, Richard G. Langlois, L. A, Azarov, D. A. Budayev, A. L. Ossipenkov, D. M. Shamayev

Author Affiliations +

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180807
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A novel biodosimetry method based on red blood cell analysis by means of laser flow cytometry and specially designed monoclonal antibodies is used for reconstructing ionizing radiation doses for Chernobyl accident victims. In addition to analysis of the patient's blood samples, we are evaluating both spectroscopy procedures and laser-based flow techniques. It was shown that resonance energy transfer processes can take place among the two different dyes links to the spherical cell surface. This can affect the accuracy of rare mutant cell scoring. Also a new technique providing conventional flow cytometric analysis and kinetic measurements of the elementary stages of biochemical reactions is described.

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Pavel G. Pleshanov, Richard G. Langlois, L. A, Azarov, D. A. Budayev, A. L. Ossipenkov, and D. M. Shamayev "Laser-based blood cell measurements for the biological reconstruction of ionizing radiation doses using a novel technique for cytometry/biochemical reaction analysis", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180807

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