Tetrasulphonated aluminum phthalocyanine as a pH-sensitive probe

Elena B. Chernyaeva; Marina Yu. Poroshina; L. V. Zhorina; A. V. Agronskaya; Andrey Yuri Chikishev; V. A. Schinov; S. Yu. Ardgantsev; Maria G. Galpern

doi:10.1117/12.199152

12 January 1995 Tetrasulphonated aluminum phthalocyanine as a pH-sensitive probe

Elena B. Chernyaeva, Marina Yu. Poroshina, L. V. Zhorina, A. V. Agronskaya, Andrey Yuri Chikishev, V. A. Schinov, S. Yu. Ardgantsev, Maria G. Galpern

Author Affiliations +

Proceedings Volume 2325, Photodynamic Therapy of Cancer II; (1995) https://doi.org/10.1117/12.199152
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

PH is a factor relevant for the molecular state of the sensitizer used in PDT and so may influence its photochemical activity and its interaction with cells and tissues. Thus the measurements of microenvironmental pH of the sensitizing molecule, its dynamics during irradiation with light and subsequent development of cell damage are interesting from the point of view of the PDT molecular and cellular mechanisms. Two ways of performing such measurements are suggested: the first one is to use conventional fluorescence or transmission pH-probes, and second using the sensitizer itself as such a probe. We show the complications arising in the first approach due to the interaction between the probing and sensitizing molecules on the example of measurements of the microenvironment pH of tetrasulphonated aluminum phthalocyanine (AlPcS₄) in cultured cells with the use of neutral red. These complications are expected to be common also for the other conventional pH-probes. We studied concentrational and pH dependencies of the fluorescence emission, fluorescence excitation and absorbance spectra, and fluorescence lifetimes of AlPcS₄ in aqueous solutions (10^-6 M - 10^-4 M) by means of stationary and laser picosecond fluorescence spectroscopies. With the use of numerical deconvolution of complex spectral curves (Alentsev - Fok algorithm) three individual components were found in AlPcS₄ pH-dependent fluorescence spectra. Based on the spectral data we suggest a ratioing technique for the local pH measurements with the use of AlPcS₄ as a fluorescence pH- sensitive probe. Microenvironmental pH of AlPcS₄ molecules in cultured cells were estimated by means of the suggested technique.

Citation Download Citation

Elena B. Chernyaeva, Marina Yu. Poroshina, L. V. Zhorina, A. V. Agronskaya, Andrey Yuri Chikishev, V. A. Schinov, S. Yu. Ardgantsev, and Maria G. Galpern "Tetrasulphonated aluminum phthalocyanine as a pH-sensitive probe", Proc. SPIE 2325, Photodynamic Therapy of Cancer II, (12 January 1995); https://doi.org/10.1117/12.199152

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Photodynamic therapy

Calibration

Molecules

Cancer

Absorbance

Absorption

Show All Keywords

Keywords/Phrases

Search In:

Publication Years