Time-resolved 3D imaging of fluorescent objects in turbid media

Jun Wu; Yang Wang; Lev T. Perelman; Irving Itzkan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1117/12.200864

31 January 1995 Time-resolved 3D imaging of fluorescent objects in turbid media

Jun Wu, Yang Wang, Lev T. Perelman, Irving Itzkan, Ramachandra R. Dasari, Michael S. Feld

Proceedings Volume 2326, Photon Transport in Highly Scattering Tissue; (1995) https://doi.org/10.1117/12.200864
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

We present a single-ended technique for three-dimensional imaging of objects embedded in a turbid medium using time-resolved fluorescence emission. The technique employs the earliest- arriving photons, which we show are not sensitive to the relatively long fluorescence lifetime and thus can be used to provide accurate spatial information even at a distance equivalent to 100 mean free paths (mfps). Experiments to combine fluorescence spectroscopy with time- resolved optical tomography are also presented. In addition, with the aid of the multichannel detection of a streak camera, we are able to accurately localize an embedded fluorescent object in a single measurement. Application of time-resolved Raman spectroscopy is also explored.

Citation Download Citation

Jun Wu, Yang Wang, Lev T. Perelman, Irving Itzkan, Ramachandra R. Dasari, and Michael S. Feld "Time-resolved 3D imaging of fluorescent objects in turbid media", Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); https://doi.org/10.1117/12.200864

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