Effect of Nd:YAG collagen synthesis by human pulp fibroblasts

Rahmat A. Barkhordar D.D.S.; Qaiser P. Ghani; Belma A. Enriquez; M. Zamir Hussain

doi:10.1117/12.238751

23 April 1996 Effect of Nd:YAG collagen synthesis by human pulp fibroblasts

Rahmat A. Barkhordar D.D.S., Qaiser P. Ghani, Belma A. Enriquez, M. Zamir Hussain

Proceedings Volume 2672, Lasers in Dentistry II; (1996) https://doi.org/10.1117/12.238751
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The effect of Nd:YAG laser on tissue is dependant on treatment power, times and frequencies. In order to determine the biologic effect Nd:YAG laser radiation on dental pulp tissue, human dental pulp fibroblasts were obtained from extracted human teeth, and grown in DME containing 10% FBS and used as passage 6 - 7. Confluent cells were exposed to defocused laser energy (1 W, 10 Hz) using a pulsed (120 microsecond(s) ) fiberoptic delivered (320 micrometers fiber) Nd:YAG for 1 - 5 minutes. The cells were labeled with 10 (mu) Ci/ml of ³H proline for 16 hours. Untreated cultures were used as controls. Collagen synthesis was expressed as DPM X 10^-3 mg/protein. The purpose of these measurements was to ascertain whether these radiation levels may induced a reparative response resulting in enhanced collagen synthesis. The results showed that at 1 - 5 minutes there was not significant effect. The tested lasing doses apparently did not cause any injurious effect to the fibroblasts, hence no increase in collagen synthesis.

Citation Download Citation

Rahmat A. Barkhordar D.D.S., Qaiser P. Ghani, Belma A. Enriquez, and M. Zamir Hussain "Effect of Nd:YAG collagen synthesis by human pulp fibroblasts", Proc. SPIE 2672, Lasers in Dentistry II, (23 April 1996); https://doi.org/10.1117/12.238751

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