Development of a genosensor for Mycobacterium tuberculosis

Narayana R. Isola; Jean Pierre Alarie; Guy D. Griffin; Tuan Vo-Dinh

doi:10.1117/12.238803

24 April 1996 Development of a genosensor for Mycobacterium tuberculosis

Narayana R. Isola, Jean Pierre Alarie, Guy D. Griffin, Tuan Vo-Dinh

Proceedings Volume 2676, Biomedical Sensing, Imaging, and Tracking Technologies I; (1996) https://doi.org/10.1117/12.238803
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

This work involves the development of a fiberoptic genosensor for the detection of Mycobacterium tuberculosis. A rapid and sensitive non-radioactive method for the detection of Mycobacterium is described. This method involves polymerase chain reaction (PCR) amplification of the target DNA utilizing gene specific primers labelled with fluorescein at the 5' end and hybridization of the amplified product to a species specific oligonucleotide probe sequence covalently linked to the end of a fiber. The fluorescent hybridization products are detected by laser induced fluorescence using an argon laser (488 nm).

Citation Download Citation

Narayana R. Isola, Jean Pierre Alarie, Guy D. Griffin, and Tuan Vo-Dinh "Development of a genosensor for Mycobacterium tuberculosis", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); https://doi.org/10.1117/12.238803

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