Microfabrication-based ektacytometer for blood cell deformability measurements

Eric H. Altendorf

doi:10.1117/12.269964

31 March 1997 Microfabrication-based ektacytometer for blood cell deformability measurements

Eric H. Altendorf

Proceedings Volume 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications; (1997) https://doi.org/10.1117/12.269964
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A unique ektacytometer for monitoring blood cell deformability constructed using a silicon and glass microfabricated flow cell, a diode laser source and CCD detector, is presented. The device described in this paper relies on the reflection of the incident laser beam from the silicon surface, and hence does not act as a transmission cell. In this sense the device is compatible with other microfabricated devices which also utilize a reflection based optical geometry. Flow induced changes in the diffraction pattern generated by blood samples flowing through the microfabricated cell, and passing through the laser beam, are analyzed and compared with expected results based on shear induced changes in erythrocyte (red blood cell) shape within the flow cell. Finally, optimization of the flow cell design, and possible applications toward biomedical instrumentation are also discussed.

Citation Download Citation

Eric H. Altendorf "Microfabrication-based ektacytometer for blood cell deformability measurements", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); https://doi.org/10.1117/12.269964

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