Laser mass spectrometry for DNA sequencing, disease diagnosis, and fingerprinting

C. H. Winston Chen; N. I. Taranenko; Y. F. Zhu; C. N. Chung; S. L. Allman

doi:10.1117/12.274339

22 May 1997 Laser mass spectrometry for DNA sequencing, disease diagnosis, and fingerprinting

C. H. Winston Chen, N. I. Taranenko, Y. F. Zhu, C. N. Chung, S. L. Allman

Proceedings Volume 2985, Ultrasensitive Biochemical Diagnostics II; (1997) https://doi.org/10.1117/12.274339
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Since laser mass spectrometry has the potential for achieving very fast DNA analysis, we recently applied it to DNA sequencing, DNA typing for fingerprinting, and DNA screening for disease diagnosis. Two different approaches for sequencing DNA have been successfully demonstrated. One is to sequence DNA with DNA ladders produced from Sanger's enzymatic method. The other is to do direct sequencing without DNA ladders. The need for quick DNA typing for identification purposes is critical for forensic application. Our preliminary results indicate laser mass spectrometry can possible be used for rapid DNA fingerprinting applications at a much lower cost than gel electrophoresis. Population screening for certain genetic disease can be a very efficient step to reducing medical costs through prevention. Since laser mass spectrometry can provide very fast DNA analysis, we applied laser mass spectrometry to disease diagnosis. Clinical samples with both base deletion and point mutation have been tested with complete success.

Citation Download Citation

C. H. Winston Chen, N. I. Taranenko, Y. F. Zhu, C. N. Chung, and S. L. Allman "Laser mass spectrometry for DNA sequencing, disease diagnosis, and fingerprinting", Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); https://doi.org/10.1117/12.274339

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