|
Comparison of lucigenin- and luminol-dependent chemiluminescence (LC-CL and LM-CL, respectively) in nondiluted healthy donors' blood revealed significant differences in their patterns. LM-CL was low in fresh blood and disappeared after it storage for 3 hours. LC-CL was already high in fresh blood and was steadily increasing with blood storage. Serial dilution of blood with saline after addition of chemiluminescence indicators resulted in elevation of LM-CL, but decrease in LC-CL. LM-CL elevation after the initiation of respiratory burst (RB) in blood with zymosan was observed only in aerated samples and immediately dropped down when air supply to a blood sample was ceased. On the contrary, LM-CL did not depend on air supply to a blood sample for about 30 min. after RB initiation. The results suggest that there are at least two mechanisms for reactive oxygen species production in nondiluted blood. The first one is reflected predominantly by LM-CL. It is activated during RB and uses prevalently oxygen dissolved in cell medium. Another one is reflected predominantly by LC- LM. It does not depend upon initiation of RB in neutrophils, operates in blood constantly, and uses oxygen supplied by erythrocytes. It needs blood integrity for its manifestation.
|
