Comparative study of skin welding in the rat using low-power CO2 laser beam: macroscopic observations and histologic and histochemical studies using Picrosirius red stain for collagen determination

Shamai Giler; Avraham Weinberger; Rivka Gal; Marissa Halpern M.D.

doi:10.1117/12.297894

14 January 1998 Comparative study of skin welding in the rat using low-power CO₂ laser beam: macroscopic observations and histologic and histochemical studies using Picrosirius red stain for collagen determination

Shamai Giler, Avraham Weinberger, Rivka Gal, Marissa Halpern M.D.

Author Affiliations +

Proceedings Volume 3195, Laser-Tissue Interaction, Tissue Optics, and Laser Welding III; (1998) https://doi.org/10.1117/12.297894
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In 50 rats, a full thickness abdominal skin incision was made with a CO₂ laser (LI) (Kaplan PenduLaser 115) and in another group, an incision was made by a scalpel (SI). These groups were divided into 2 subgroups: a low power CO₂ laser skin welding of 500 mw was used in LI and SI subgroups and, in the other, the wound was closed with nylon sutures. On the fourth postoperative day in the LI welding group, a complete dehiscence wound was formed in one animal and in the SI welding group, a partial dehiscence. After one week, a thin line of young (fresh) scar was observed with complete healing after 2 weeks. Histology revealed after four days, a deep cleft in the LI and SI welding groups and a superficial ulcer after one week and after two weeks, all groups showed complete healing. Histochemical studies (Picrosirius red stain) revealed in the first two weeks, greenish-yellow polarized colors and red-orange colors of a matured collagen in the well-formed scar. It appears that there was no delay in the healing process between the LI and SI groups and between welding and suture healing.

Citation Download Citation

Shamai Giler, Avraham Weinberger, Rivka Gal, and Marissa Halpern M.D. "Comparative study of skin welding in the rat using low-power CO₂ laser beam: macroscopic observations and histologic and histochemical studies using Picrosirius red stain for collagen determination", Proc. SPIE 3195, Laser-Tissue Interaction, Tissue Optics, and Laser Welding III, (14 January 1998); https://doi.org/10.1117/12.297894

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Collagen

Lithium

Carbon dioxide lasers

Skin

Wound healing

Laser tissue interaction

Tissues

Show All Keywords

Keywords/Phrases

Search In:

Publication Years