Implantation of microfabricated immunoisolating biocapsules

Tejal A. Desai; Wen Hwa Chu; Mauro Ferrari; Guido Rasi; Paola Sinibaldi-Vallebona; Patrizia Borboni; G. Beattie; A. Hayek

doi:10.1117/12.304387

26 March 1998 Implantation of microfabricated immunoisolating biocapsules

Tejal A. Desai, Wen Hwa Chu, Mauro Ferrari, Guido Rasi, Paola Sinibaldi-Vallebona, Patrizia Borboni, G. Beattie, A. Hayek

Author Affiliations +

Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304387
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Silicon-based biocapsules have been microfabricated with uniform and well-controlled pore dimensions in the tens of nanometer range to provide effective immunoisolation of cell xenografts. Surface and bulk micromachining were integrated in the fabrication process, resulting in a diffusion membrane with mechanical and chemical stability, surrounded by an anisotropically-etched silicon wafer, which serves as the encapsulation cavity. The membrane allows the diffusion of essential nutrients while blocking the passage of immune molecules, which may destroy cellular transplants. Preliminary short term studies on both primary pancreatic islets and insulinoma cell lines encapsulated within microfabricated biocapsules were conducted to determine the toxicity and biocompatibility of biocapsules, the viability and functionality of encapsulated cells, as well as the overall immunoprotective capabilities of the biocapsule. Results seem to indicate that microfabricated biocapsules are non-toxic and do not elicit any adverse inflammatory reactions when implanted. Furthermore, encapsulated insulinoma cells remained viable and functional within microfabricated environments in vivo. These results show the feasibility and potential application of microfabricated biocapsules for several pathologies.

Citation Download Citation

Tejal A. Desai, Wen Hwa Chu, Mauro Ferrari, Guido Rasi, Paola Sinibaldi-Vallebona, Patrizia Borboni, G. Beattie, and A. Hayek "Implantation of microfabricated immunoisolating biocapsules", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304387

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available