Application and calibration of a field-portable excitation-emission matrix fluorometer for analysis of environmental contaminants

Renee D. JiJi; Michelle L. Nahorniak; Evert Fruitman; Karl S. Booksh

doi:10.1117/12.372888

16 December 1999 Application and calibration of a field-portable excitation-emission matrix fluorometer for analysis of environmental contaminants

Renee D. JiJi, Michelle L. Nahorniak, Evert Fruitman, Karl S. Booksh

Author Affiliations +

Proceedings Volume 3854, Pattern Recognition, Chemometrics, and Imaging for Optical Environmental Monitoring; (1999) https://doi.org/10.1117/12.372888
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

A field portable, single measurement, excitation-emission matrix fluorometer is applied to five environmental analyses. All applications require the uses of multi-way spectral deconvolution and calibration methods in order to uniquely resolve the spectral profiles of the targets analytes from interfering fluorophores. A modified multi-way calibration methods are applied to extend the dynamic range of the fluorometer by permitting robust calibration in the presence of intense Rayleigh and Raman backgrounds and localized detector saturation. The multi-way resolution methods are applied to calibrate chemically induced fluorescence changes induced by a target analyte from that of a fluorescent probe designed for molecular recognition. The multi-way resolution and calibration methods are also applied to potential sensor design relying of photoinduced degradation to and form fluorescent products. The EEM and multi-way methods are finally demonstrated in determining binding constants and capacity factors for fluorescent metal-ligand interactions.

Citation Download Citation

Renee D. JiJi, Michelle L. Nahorniak, Evert Fruitman, and Karl S. Booksh "Application and calibration of a field-portable excitation-emission matrix fluorometer for analysis of environmental contaminants", Proc. SPIE 3854, Pattern Recognition, Chemometrics, and Imaging for Optical Environmental Monitoring, (16 December 1999); https://doi.org/10.1117/12.372888

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