Application of laser microbeam in biopharmacy

Yansheng Zhao; Ming Wang; Luyan Wang; Taiming Li; Junmin Wu; Lei Xu; Xi Zhu

doi:10.1117/12.364349

17 September 1999 Application of laser microbeam in biopharmacy

Yansheng Zhao, Ming Wang, Luyan Wang, Taiming Li, Junmin Wu, Lei Xu, Xi Zhu

Proceedings Volume 3863, 1999 International Conference on Biomedical Optics; (1999) https://doi.org/10.1117/12.364349
Event: International Symposium on Biomedical Optics, 1999, Wuhan, China

Abstract

Laser microbeam system consists of a third harmonic generation Nd:YAG laser and an inverted biological microscope. It is the first time that laser microbeam is applied in immobilized cell to produce midecamycin (MDM). After laser irradiation, the penetrance of immobilized granule greatly increased, probably through the microchannel made by laser. The results showed that the MDM productivity was increased greatly and the half-life of immobilized mycelia prolonged over three times as long as that of the control. In another experiment, plasmid pS65T (including green fluorescent protein genes) and plasmid pUH-10 (including Amp resistant gene) were successfully introduced into E. coli by laser microbeam. The results show that laser microbeam has a bright perspective on biopharmacy.

Citation Download Citation

Yansheng Zhao, Ming Wang, Luyan Wang, Taiming Li, Junmin Wu, Lei Xu, and Xi Zhu "Application of laser microbeam in biopharmacy", Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); https://doi.org/10.1117/12.364349

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