Blood cell counting and classification by nonflowing laser light scattering method

Ye Yang; Zhenxi Zhang; Xinhui Yang; Dazong Jiang; Joon Hock Yeo

doi:10.1117/12.369297

11 November 1999 Blood cell counting and classification by nonflowing laser light scattering method

Ye Yang, Zhenxi Zhang, Xinhui Yang, Dazong Jiang, Joon Hock Yeo

Proceedings Volume 3897, Advanced Photonic Sensors and Applications; (1999) https://doi.org/10.1117/12.369297
Event: International Symposium on Photonics and Applications, 1999, Singapore, Singapore

Abstract

A new non-flowing laser light scattering method for counting and classifying blood cells is presented. A linear charge- coupled device with 1024 elements is used to detect the scattered light intensity distribution of the blood cells. A pinhole plate is combined with the CCD to compete the focusing of the measurement system. An isotropic sphere is used to simulate the blood cell. Mie theory is used to describe the scattering of blood cells. In order to inverse the size distribution of blood cells from their scattered light intensity distribution, Powell method combined with precision punishment method is used as a dependent model method for measurement red blood cells and blood plates. Non-negative constraint least square method combined with Powell method and precision punishment method is used as an independent model for measuring white blood cells. The size distributions of white blood cells and red blood cells, and the mean diameter of red blood cells are measured by this method. White blood cells can be divided into three classes: lymphocytes, middle-sized cells and neutrocytes according to their sizes. And the number of blood cells in unit volume can also be measured by the linear dependence of blood cells concentration on scattered light intensity.

Citation Download Citation

Ye Yang, Zhenxi Zhang, Xinhui Yang, Dazong Jiang, and Joon Hock Yeo "Blood cell counting and classification by nonflowing laser light scattering method", Proc. SPIE 3897, Advanced Photonic Sensors and Applications, (11 November 1999); https://doi.org/10.1117/12.369297

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