Raman and/or surface-enhanced Raman: advantages and limitations when applied for confocal multispectral imaging with living cells

Igor Chourpa; Serguei Charonov; Michel Manfait

doi:10.1117/12.384960

8 May 2000 Raman and/or surface-enhanced Raman: advantages and limitations when applied for confocal multispectral imaging with living cells

Igor Chourpa, Serguei Charonov, Michel Manfait

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Proceedings Volume 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques; (2000) https://doi.org/10.1117/12.384960
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The in situ investigations of living cells are among the most exciting biomedical applications of the confocal multispectral imaging (CMSI). The latter is often noted as chemical imaging since provides structurally specific information. This allows non-invasive mapping of molecular components of the cells or intracellular distribution of small Raman-active chromophores such as drugs, ion-markers, etc. Here, we attempt a comparative analysis of the major advantages and limitations of surface-enhanced Raman (SER) and non-enhanced Raman techniques as used for intracellular CMSI. Based on our experimental observations and on recent data from literature we conclude that, depending on application, the differences between the two methods can be dramatic and a choice of the proper approach is imposed during acquisition, treatment and interpretation. Moreover, while Raman and SER signal can be present on the same image, these however should be treated separately, in the different way. We discuss particularities of the mapping algorithms we use to generate Raman/SER images. Concerning SER, we compare different SER-active substrates in terms of imaging on cells, taking into account both principal and practical questions.

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Igor Chourpa, Serguei Charonov, and Michel Manfait "Raman and/or surface-enhanced Raman: advantages and limitations when applied for confocal multispectral imaging with living cells", Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); https://doi.org/10.1117/12.384960

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KEYWORDS

Raman spectroscopy

Surface enhanced Raman spectroscopy

Confocal microscopy

Silver

Chromophores

Imaging spectroscopy

Metals

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