Ca2+ homeostasis in microvascular endothelial cells from an insulin-dependent diabetic model: role of endosomes/lysosomes

Shankar C. Sanka; David C. Bennett; Jose D. Rojas; Geraldine B. Tasby; Cynthia J. Meininger; Guoyao Wu; Donald E. Wesson; Curtis Michael Pfarr; Raul Martinez-Zaguilan

doi:10.1117/12.384240

26 April 2000 Ca²⁺homeostasis in microvascular endothelial cells from an insulin-dependent diabetic model: role of endosomes/lysosomes

Shankar C. Sanka, David C. Bennett, Jose D. Rojas, Geraldine B. Tasby, Cynthia J. Meininger, Guoyao Wu, Donald E. Wesson, Curtis Michael Pfarr, Raul Martinez-Zaguilan

Author Affiliations +

Proceedings Volume 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation; (2000) https://doi.org/10.1117/12.384240
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Cytosolic Ca²⁺ ([Ca²⁺]^cyt) regulates several cellular functions, e.g. cell growth, contraction, secretion, etc. In many cell types, ion homeostasis appears to be coupled with glucose metabolism. In certain cell types, a strict coupling between glycolysis and the activity of Sarcoplasmic/Endoplasmic Reticulum Ca²⁺-ATPases (SERCA) has been suggested. Glucose metabolism is altered in diabetes. We hypothesize that: (1) Ca²⁺ homeostasis is altered in microvascular endothelial cells from diabetic animals due to the dysfunction of glycolysis coupling the activity of SERCA; (2) endosomal/lysosomal compartments expressing SERCA are involved in the dysfunction associated with diabetes.

Citation Download Citation

Shankar C. Sanka, David C. Bennett, Jose D. Rojas, Geraldine B. Tasby, Cynthia J. Meininger, Guoyao Wu, Donald E. Wesson, Curtis Michael Pfarr, and Raul Martinez-Zaguilan "Ca²⁺homeostasis in microvascular endothelial cells from an insulin-dependent diabetic model: role of endosomes/lysosomes", Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); https://doi.org/10.1117/12.384240

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available