High-speed high-resolution epifluorescence imaging system using CCD sensor and digital storage for neurobiological research

Ichiro Takashima; Riichi Kajiwara; Kiyo Murano; Toshio Iijima; Yasuhiro Morinaka; Hiroyoshi Komobuchi

doi:10.1117/12.424259

17 April 2001 High-speed high-resolution epifluorescence imaging system using CCD sensor and digital storage for neurobiological research

Ichiro Takashima, Riichi Kajiwara, Kiyo Murano, Toshio Iijima, Yasuhiro Morinaka, Hiroyoshi Komobuchi

Author Affiliations +

Proceedings Volume 4183, 24th International Congress on High-Speed Photography and Photonics; (2001) https://doi.org/10.1117/12.424259
Event: 24th International Congress on High-Speed Photography and Photonics, 2000, Sendai, Japan

Abstract

We have designed and built a high-speed CCD imaging system for monitoring neural activity in an exposed animal cortex stained with a voltage-sensitive dye. Two types of custom-made CCD sensors were developed for this system. The type I chip has a resolution of 2664 (H) X 1200 (V) pixels and a wide imaging area of 28.1 X 13.8 mm, while the type II chip has 1776 X 1626 pixels and an active imaging area of 20.4 X 18.7 mm. The CCD arrays were constructed with multiple output amplifiers in order to accelerate the readout rate. The two chips were divided into either 24 (I) or 16 (II) distinct areas that were driven in parallel. The parallel CCD outputs were digitized by 12-bit A/D converters and then stored in the frame memory. The frame memory was constructed with synchronous DRAM modules, which provided a capacity of 128 MB per channel. On-chip and on-memory binning methods were incorporated into the system, e.g., this enabled us to capture 444 X 200 pixel-images for periods of 36 seconds at a rate of 500 frames/second. This system was successfully used to visualize neural activity in the cortices of rats, guinea pigs, and monkeys.

Citation Download Citation

Ichiro Takashima, Riichi Kajiwara, Kiyo Murano, Toshio Iijima, Yasuhiro Morinaka, and Hiroyoshi Komobuchi "High-speed high-resolution epifluorescence imaging system using CCD sensor and digital storage for neurobiological research", Proc. SPIE 4183, 24th International Congress on High-Speed Photography and Photonics, (17 April 2001); https://doi.org/10.1117/12.424259

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
12 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Charge-coupled devices

Imaging systems

Field programmable gate arrays

Optical imaging

Cameras

Brain

Head

Show All Keywords

Keywords/Phrases

Search In:

Publication Years