Suitable microscope objectives for multiphoton digital imaging

Gerald S. Benham; Stanley Schwartz

doi:10.1117/12.470673

17 June 2002 Suitable microscope objectives for multiphoton digital imaging

Gerald S. Benham, Stanley Schwartz

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470673
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The proper choice of an objective lens can have a profound effect on multiphoton microscopy. Multiphoton requires that the objective have adequate transmission in the IR range. This technology has a requisite to image the specimen near the slide surface next to the cover glass and to of 200 microns or more. Fluor and Plan Apochromat objectives. All lenses suffer from aberrations, which can prevent imaging an exact replica of the object. Most objectives could be used for multiphoton imaging;; however lenses should be selected from Plan Fluors, Plan Apochromats, and Super (S) Fluors. Highly corrected water immersion objectives (WI) permit deep penetration within aqueous environments and living tissues, beyond that of oil immersion objectives. Spectral transmission data is presented for Nikon CFI60 various objective lenses with high Nas, long working distances, and maximum aberration correction.

Citation Download Citation

Gerald S. Benham and Stanley Schwartz "Suitable microscope objectives for multiphoton digital imaging", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470673

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