Nonlinear phase contrast using a bacteriorhodopsin film

Marcelo David Iturbe Castillo; J. David Sanchez-de-la-Llave; Ruben Ramos Garcia; Eduardo Tepichin-Rodriguez; L. I. Olivos-Perez

doi:10.1117/12.456519

27 November 2002 Nonlinear phase contrast using a bacteriorhodopsin film

Marcelo David Iturbe Castillo, J. David Sanchez-de-la-Llave, Ruben Ramos Garcia, Eduardo Tepichin-Rodriguez, L. I. Olivos-Perez

Author Affiliations +

Proceedings Volume 4789, Algorithms and Systems for Optical Information Processing VI; (2002) https://doi.org/10.1117/12.456519
Event: International Symposium on Optical Science and Technology, 2002, Seattle, WA, United States

Abstract

In this paper we demonstrate a novel phase contrast system that employs a BR film. Since the filter is optically induced by the Fourier transform of the phase object, no alignment is necessary at the filter plane making it extremely robust. Due to the optical properties of BR films the phase filter can be induced with low light intensity levels. The material response allows operation at video frame rates, processing of high spatial resolution objects, and the use of relatively inexpensive laser sources. Such characteristics and the fact that BR films can be produced at a low cost makes the system simple to implement, relatively inexpensive and extremely robust. The effects of varying the illuminating area beyond the phase object area and filter saturation are also analyzed.

Citation Download Citation

Marcelo David Iturbe Castillo, J. David Sanchez-de-la-Llave, Ruben Ramos Garcia, Eduardo Tepichin-Rodriguez, and L. I. Olivos-Perez "Nonlinear phase contrast using a bacteriorhodopsin film", Proc. SPIE 4789, Algorithms and Systems for Optical Information Processing VI, (27 November 2002); https://doi.org/10.1117/12.456519

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