Fluorescence apertureless scanning near-field microscopy for high-resolution biological imaging

Alexandra Fragola; Lionel Aigouy

doi:10.1117/12.500667

14 October 2003 Fluorescence apertureless scanning near-field microscopy for high-resolution biological imaging

Alexandra Fragola, Lionel Aigouy

Proceedings Volume 5143, Novel Optical Instrumentation for Biomedical Applications; (2003) https://doi.org/10.1117/12.500667
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Apertureless scanning near-field optical microscopy (SNOM) offers new opportunities in fluorescence imaging by providing subwavelength resolution. This is achieved by scattering the near-field with a metallic tip. SNOM images have been recorded on fluorescent spheres and erbium-doped vitroceramic. We will also present approach curves that allow to better understand the near-field optical contrast origin. Our near-field microscope is now suitable for immersed samples imaging, in order to study biological samples.

Citation Download Citation

Alexandra Fragola and Lionel Aigouy "Fluorescence apertureless scanning near-field microscopy for high-resolution biological imaging", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); https://doi.org/10.1117/12.500667

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