In vitro cytotoxicity assessment of [5,10,15,20-tetra (4-sulfophenyl) porphyrin] on tumor and nontumor cell lines

R. Alexandrova; O. Sabotinov; Elena V. Stoykova; Rodica-Mariana Ion; Stanislav Shurulinkov; Georgi Minchev

doi:10.1117/12.563137

21 June 2004 In vitro cytotoxicity assessment of [5,10,15,20-tetra (4-sulfophenyl) porphyrin] on tumor and nontumor cell lines

R. Alexandrova, O. Sabotinov, Elena V. Stoykova, Rodica-Mariana Ion, Stanislav Shurulinkov, Georgi Minchev

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Proceedings Volume 5449, Eighth International Conference on Laser and Laser Information Technologies; (2004) https://doi.org/10.1117/12.563137
Event: Eighth International Conference on Laser and Laser Information Technologies, 2003, Smolyan, Bulgaria

Abstract

In this study we evaluate the cytotoxicity of 5,10,15,20- tetra (4-sulfophenyl) porphyrins on a tumor cell line LSCC-SF-Mc29, obtained from a transplantable chicken hepatoma induced by the myelocytomatosis virus Mc29, a timor line LSR-SF-SR, obtained from a transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin and for normal mouse cell line (BALB/c-3T3-A31) and bovine kidney cell line (MDBK). The cells were exposed to irradiation from a pulsed CuBr vapor laser system at 510.6 nm and 578.2 nm at fluence rate 50 mW/cm² and pulse frequency rate 20 kHz. The viability of cells was determined by the neutral red uptake cytotoxicity assay. The light dose-response curves and light exposures that ensure viability drop to 50 % were obtained for each cell line. The cytotoxic effect of TS₄PP is most distinguished for LSCC-SF-Mc29. The bovine cell line is more vulnerable than the mouse line, especially at 510.6 nm. The 2-4 times higher viability of the normal cell lines in comparison with the tumor lines has been obtained.

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R. Alexandrova, O. Sabotinov, Elena V. Stoykova, Rodica-Mariana Ion, Stanislav Shurulinkov, and Georgi Minchev "In vitro cytotoxicity assessment of [5,10,15,20-tetra (4-sulfophenyl) porphyrin] on tumor and nontumor cell lines", Proc. SPIE 5449, Eighth International Conference on Laser and Laser Information Technologies, (21 June 2004); https://doi.org/10.1117/12.563137

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