A new method of quantitative determination of apoptotic parameters in cellular suspensions

Olexander I. Bilyi; Rostyslav O. Bilyy; Vasyl B. Getman

doi:10.1117/12.560052

4 June 2004 A new method of quantitative determination of apoptotic parameters in cellular suspensions

Olexander I. Bilyi, Rostyslav O. Bilyy, Vasyl B. Getman

Proceedings Volume 5477, Sixth International Conference on Correlation Optics; (2004) https://doi.org/10.1117/12.560052
Event: Sixth International Conference on Correlation Optics, 2003, Chernivsti, Ukraine

Abstract

In report a new method of quantitative determination of apoptosis parameters in cellular suspensions using correlation optic methods is considered. The principle of the new optical verification method is described. This method includes sounding of liquid flow with monochromatic coherent light, registration of impulse signals resulting from interaction of sounding radiation with cells, and measuring impulses' amplitude and duration and construction on the basis of obtained measurement results functions of two-dimensional pulses' amplitudes and duration distribution that express statistical intensity characteristics of light scattering by cells. On the basis of obtained functions the size distribution of cells is determined and apoptosis parameters are estimated. Results of apoptotic cells of K562 line (human leukemia), induced by cisplatin action, are described.

Citation Download Citation

Olexander I. Bilyi, Rostyslav O. Bilyy, and Vasyl B. Getman "A new method of quantitative determination of apoptotic parameters in cellular suspensions", Proc. SPIE 5477, Sixth International Conference on Correlation Optics, (4 June 2004); https://doi.org/10.1117/12.560052

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