Applications of small surface plasmon resonance sensors for biochemical monitoring

Jean-Francois Masson; Tina M. Battaglia; Stephen Beaudoin; Karl S. Booksh

doi:10.1117/12.571522

7 December 2004 Applications of small surface plasmon resonance sensors for biochemical monitoring

Jean-Francois Masson, Tina M. Battaglia, Stephen Beaudoin, Karl S. Booksh

Proceedings Volume 5588, Smart Medical and Biomedical Sensor Technology II; (2004) https://doi.org/10.1117/12.571522
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

The development of small surface plasmon resonance (SPR) sensors to detect biological markers for myocardial ischemia (MI), spinal muscular atrophy (SMA), and wound healing was achieved at low ng/mL and in less than 10 minutes. The markers of interest for MIs are myoglobin (MG) and cardiac Troponin I (cTnI). The limits of detection for these markers are respectively 600 pg/mL and 1.4 ng/mL in saline solution. To study SMA, the level of survival motor neuron protein (SMN) was investigated. A limit of detection of 990 pg/mL was achieved for the detection of SMN. The interactions of SMN with MG decreased the signal for both SMN and MG. Interleukin 6 and tumor necrosis factor alpha (TNFa) were investigated to monitor wound healing. The sensor's performance in more complex solutions, e.g.: serum, showed a large non-specific signal. Modifying the support on which the antibodies are attached improved the sensor's stability in serum by a factor of 5. To achieve this non-specific binding (NSB) reduction, different polysaccharides, biocompatible polymers and short chain thiols were investigated.

Citation Download Citation

Jean-Francois Masson, Tina M. Battaglia, Stephen Beaudoin, and Karl S. Booksh "Applications of small surface plasmon resonance sensors for biochemical monitoring", Proc. SPIE 5588, Smart Medical and Biomedical Sensor Technology II, (7 December 2004); https://doi.org/10.1117/12.571522

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