Differential phase surface plasmon resonance biosensors

Ho Pui Ho; Shu Yuen Wu; Chinlon Lin

doi:10.1117/12.581160

18 January 2005 Differential phase surface plasmon resonance biosensors

Ho Pui Ho, Shu Yuen Wu, Chinlon Lin

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.581160
Event: Photonics Asia, 2004, Beijing, China

Abstract

An optical differential phase surface plasmon resonance (SPR) technique capable of performing refractive index measurement with accuracy in the order of 5 x 10^-8 is presented. The system makes use of the s-polarization as the reference beam to interfere with the p-polarization, of which the phase has close relationship with the change of surface plasmon resonance conditions at the sensor surface. The extraction of phase information is achieved by performing fringe analysis on the interference pattern captured by a digital oscilloscope. Results obtained from monitoring BSA (Bovine Serum Albumin) binding reaction with BSA antibodies demonstrated that our setup has a sensitivity limit of 7.4 ng ml^-1.

Citation Download Citation

Ho Pui Ho, Shu Yuen Wu, and Chinlon Lin "Differential phase surface plasmon resonance biosensors", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.581160

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