Two-photon fluorescence microscope with a hollow-core photonic crystal fiber

Shih-Peng Tai; Ming-Che Chan; Tsung-Han Tsai; Shi-Hao Guol; Li-Jin Chen; Chi-Kuang Sun

doi:10.1117/12.588474

23 March 2005 Two-photon fluorescence microscope with a hollow-core photonic crystal fiber

Shih-Peng Tai, Ming-Che Chan, Tsung-Han Tsai, Shi-Hao Guol, Li-Jin Chen, Chi-Kuang Sun

Proceedings Volume 5691, Optical Fibers and Sensors for Medical Applications V; (2005) https://doi.org/10.1117/12.588474
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Since the first demonstration in 1990, two-photon fluorescence microscopy (TPFM) has made a great impact on biomedical researches. With its high penetration ability, low out-of-focus photodamage, and intrinsic three-dimensional (3D) sectioning capability, TPFM has been widely applied to various medical diagnosis and genome researches. Recently, single-mode optical fibers were introduced into the TPFM systems for remote optical pulse delivery. Fiber-based TPFM has advantages including isolating the vibration from laser and electronic devices, flexible system design, and low cross-talks. It is also the first step toward an all-fiber based two-photon endoscope. However, due to serious temporal broadening when conventional Ti:sapphire based femtosecond pulses propagate through the fiber, the two-photon excitation efficiency of the fiber-optic TPFM is much lower than the conventional one. The temporal broadening effect mainly comes from group velocity dispersion (GVD) and self-phase modulation (SPM), which also leads to significant spectral broadening. To reduce the temporal broadening effect, here we present a hollow-core photonic-bandgap fiber based TPFM. By replacing the conventional single-mode fiber with the hollow core photonic bandgap fiber, the GVD and SPM effects can be greatly reduced for high intensity, ultra-short pulse delivery. Femtosecond Ti:sapphire pulses passing through the fiber with negligible GVD and SPM effects is demonstrated in this paper. Much improvement of two-photon fluorescence excitation efficiency is thus achieved with the hollow-core photonic-bandgap fiber based TPFM.

Citation Download Citation

Shih-Peng Tai, Ming-Che Chan, Tsung-Han Tsai, Shi-Hao Guol, Li-Jin Chen, and Chi-Kuang Sun "Two-photon fluorescence microscope with a hollow-core photonic crystal fiber", Proc. SPIE 5691, Optical Fibers and Sensors for Medical Applications V, (23 March 2005); https://doi.org/10.1117/12.588474

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available