14 September 2006The interaction of lipopolysaccharide-coated polystyrene particle with membrane receptor proteins on macrophage measured by optical tweezers
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Lipopolysaccharide (LPS) is one of the cell wall components of Gram-positive bacteria recognized by
and interacted with receptor proteins such as CD14 on macrophage cells. Such a process plays an
important role in our innate immune system. In this paper, we report the application of optical tweezers
(λ = 1064nm Gaussian beam focused by a water-immersed objective lens with N.A. = 1.0) to the study
of the dynamics of the binding of a LPS-coated polystyrene particle (diameter = 1.5μm) onto the
plasma membrane of a macrophage cell. We demonstrated that the binding rate increased significantly
when the macrophage cell was pre-treated with the extract of Reishi polysaccharides (EORP) which
has been shown to enhance the cell surface expression of CD14 (receptor of LPS) on macrophage cells.
Ming-Tzo Wei,Kuo-Feng Hua,Jowey Hsu,Artashes Karmenyan,Hsien-Yeh Hsu, andArthur Chiou
"The interaction of lipopolysaccharide-coated polystyrene particle with membrane receptor proteins on macrophage measured by optical tweezers", Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63260T (14 September 2006); https://doi.org/10.1117/12.679258
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Ming-Tzo Wei, Kuo-Feng Hua, Jowey Hsu, Artashes Karmenyan, Hsien-Yeh Hsu, Arthur Chiou, "The interaction of lipopolysaccharide-coated polystyrene particle with membrane receptor proteins on macrophage measured by optical tweezers," Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63260T (14 September 2006); https://doi.org/10.1117/12.679258