Fluorescence intermittency and spectral shifts of single bio-conjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy

HyeongGon Kang; Mathew M. Maye; Dmytro Nykypanchuk; Matthew Clarke; Peter Yim; Jeffrey Krogmeier; Kimberly Briggman; Oleg Gang; Jeeseong Hwang

doi:10.1117/12.713934

7 February 2007 Fluorescence intermittency and spectral shifts of single bio-conjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy

HyeongGon Kang, Mathew M. Maye, Dmytro Nykypanchuk, Matthew Clarke, Peter Yim, Jeffrey Krogmeier, Kimberly Briggman, Oleg Gang, Jeeseong Hwang

Author Affiliations +

Proceedings Volume 6430, Advanced Biomedical and Clinical Diagnostic Systems V; 643024 (2007) https://doi.org/10.1117/12.713934
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We have fabricated a combined measurement system capable of confocal microscopy and fluorescence spectroscopy to simultaneously evaluate multiple optical characteristics of single fluorescent nanocrystals. The single particle detection sensitivity is demonstrated by simultaneously measuring the dynamic excitation-time-dependent fluorescence intermittency and the emission spectrum of single cadmium selenide/zinc sulfide (CdSe/ZnS) nanocrystals (quantum dots, QDs). Using this system, we are currently investigating the optical characteristics of single QDs, the surface of which are conjugated with different ligands, such as trioctylphosphine oxide (TOPO), mercaptoundecanoicacid (MDA), and amine modified DNA (AMDNA). In this paper, we present the progress of our measurements of the time-dependent optical characteristics (fluorescence intermittency, photostability, and spectral diffusion) of single MDA-QDs and AMDNA-MDA-QDs in air in an effort to understand the effects of surface-conjugated biomolecules on the optical characteristics at single QD sensitivities.

Citation Download Citation

HyeongGon Kang, Mathew M. Maye, Dmytro Nykypanchuk, Matthew Clarke, Peter Yim, Jeffrey Krogmeier, Kimberly Briggman, Oleg Gang, and Jeeseong Hwang "Fluorescence intermittency and spectral shifts of single bio-conjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy", Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 643024 (7 February 2007); https://doi.org/10.1117/12.713934

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