Two-colour microparticle detection in PDMS biochips with integrated optics

C. L. Bliss; C. J. Backhouse; J. N. McMullin

doi:10.1117/12.778513

30 October 2007 Two-colour microparticle detection in PDMS biochips with integrated optics

C. L. Bliss, C. J. Backhouse, J. N. McMullin

Proceedings Volume 6796, Photonics North 2007; 67960A (2007) https://doi.org/10.1117/12.778513
Event: Photonics North 2007, 2007, Ottawa, Canada

Abstract

It is demonstrated how cells that respond to different wavelengths of excitation laser light in a lab-on-a-chip device may be uniquely identified using only a single sensitive photodetector. The technique used is to apply modulation to the excitation lasers and test for the modulation in the emitted optical signals. A simple sliding-window Fourier transform algorithm is applied to the streaming data to eliminate all but the modulation frequency of interest. The method is demonstrated in principle by detecting and identifying two different types of fluorescent polystyrene beads in a pressure-driven flow in a microchannel

Citation Download Citation

C. L. Bliss, C. J. Backhouse, and J. N. McMullin "Two-colour microparticle detection in PDMS biochips with integrated optics", Proc. SPIE 6796, Photonics North 2007, 67960A (30 October 2007); https://doi.org/10.1117/12.778513

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