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A method is proposed for determining blood oxygen saturation in frozen tissue. The method is based on a spectral
camera system equipped with an Acoustic-Optical-Tuneable-Filter. The HSI-setup is validated by measuring series of
unfrozen and frozen samples of a hemoglobin-solution, a hemoglobin-intralipid mixture and whole blood with varying
oxygen saturation. The theoretically predicted linear relation between oxygen saturation and absorbance was observed in
both the frozen sample series and the unfrozen series. In a final proof of principal, frozen myocardial tissue was
measured. Higher saturation values were recorded for ventricle and atria tissue compared to the septum and connective
tissue. These results are not validated by measurements with another method. The formation of methemoglobin during
freezing and the presence of myoglobin in the tissue turned out to be possible sources of error.
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Boy Braaf, Annemarie Nadort, Dirk Faber, Rene ter Wee, Ton van Leeuwen, Maurice Aalders, "Blood oxygen saturation of frozen tissue determined by hyper spectral imaging," Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 685907 (18 March 2008); https://doi.org/10.1117/12.763027