Confocal light scattering and absorption spectroscopic microscopy

Le Qiu; Edward Vitkin; Saira Salahuddin; Munir M. Zaman; Charlotte Andersson; Steven D. Freedman; Eugene B. Hanlon; Irving Itzkan; Lev T. Perelman

doi:10.1117/12.781562

2 May 2008 Confocal light scattering and absorption spectroscopic microscopy

Le Qiu, Edward Vitkin, Saira Salahuddin, Munir M. Zaman, Charlotte Andersson, Steven D. Freedman, Eugene B. Hanlon, Irving Itzkan, Lev T. Perelman

Author Affiliations +

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69910X (2008) https://doi.org/10.1117/12.781562
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

We have developed a novel optical method for observing submicron intracellular structures in living cells which is called confocal light absorption and scattering spectroscopic (CLASS) microscopy. It combines confocal microscopy, a well-established high-resolution microscopic technique, with light scattering spectroscopy (LSS). CLASS microscopy requires no exogenous labels and is capable of imaging and continuously monitoring individual viable cells, enabling the observation of cell and organelle functioning at scales on the order of 100 nm. In addition, it provides not only size information but also information about the biochemical and physical properties of the cell.

Citation Download Citation

Le Qiu, Edward Vitkin, Saira Salahuddin, Munir M. Zaman, Charlotte Andersson, Steven D. Freedman, Eugene B. Hanlon, Irving Itzkan, and Lev T. Perelman "Confocal light scattering and absorption spectroscopic microscopy", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910X (2 May 2008); https://doi.org/10.1117/12.781562

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