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Laser/microscope fluorescence methods for studying adsorption/desorption kinetics and surface diffusion of molecules reversibly associated with biological membranes are discussed. These methods, which include fluorescence excitation by evanescent waves generated by total internal reflection, and fluorescence quenching by thin metal films, may also find application in the design of in vivo biosensors and in studies of transmembrane transport and chemical kinetics.
Daniel Axelrod,Edward H. Hellen, andRobert M. Fulbright
"Biomolecular Motion On Surfaces And Membranes", Proc. SPIE 0712, Lasers in Medicine, (10 March 1987); https://doi.org/10.1117/12.937331
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Daniel Axelrod, Edward H. Hellen, Robert M. Fulbright, "Biomolecular Motion On Surfaces And Membranes," Proc. SPIE 0712, Lasers in Medicine, (10 March 1987); https://doi.org/10.1117/12.937331