Merocyanine-540 mediated photodynamic effects on staphylococcus epidermidis biofilms

Maria Sonia Sbarra; Antonella Di Poto; Enrica Saino; Livia Visai; Paolo Minzioni; Francesca Bragheri; Ilaria Cristiani

doi:10.1117/12.831889

17 July 2009 Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms

Maria Sonia Sbarra, Antonella Di Poto, Enrica Saino, Livia Visai, Paolo Minzioni, Francesca Bragheri, Ilaria Cristiani

Proceedings Volume 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV; 73730R (2009) https://doi.org/10.1117/12.831889
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).

Citation Download Citation

Maria Sonia Sbarra, Antonella Di Poto, Enrica Saino, Livia Visai, Paolo Minzioni, Francesca Bragheri, and Ilaria Cristiani "Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms", Proc. SPIE 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV, 73730R (17 July 2009); https://doi.org/10.1117/12.831889

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