Three-dimensional image formation under single-photon ultra-short pulsed illumination

Arijit Kumar De; Debabrata Goswami

doi:10.1117/12.822773

22 May 2009 Three-dimensional image formation under single-photon ultra-short pulsed illumination

Arijit Kumar De, Debabrata Goswami

Proceedings Volume 7378, Scanning Microscopy 2009; 737827 (2009) https://doi.org/10.1117/12.822773
Event: SPIE Scanning Microscopy, 2009, Monterey, California, United States

Abstract

The major thrust of modern day fluorescence laser-scanning microscopy have been towards achieving better and better depth resolution embodied by the invention and subsequent development of confocal and multi-photon microscopic techniques. However, each method bears its own limitations: in having sufficient background fluorescence and photodamage resulting from out-of-focus illumination for the former, while low multi-photon absorption cross-sections of common fluorophores for the latter. Here we show how the intelligent choice of single-photon ultrashort pulsed illumination can circumvent all these shortcomings by exemplifying the tiny spatial stretch of an ultrashort pulse. Besides achieving a novel way of optical sectioning, this new method offers improved signal-to-noise ratio as well as reduced photo-damage which are crucial for live cell imaging under prolonged exposure to light.

Citation Download Citation

Arijit Kumar De and Debabrata Goswami "Three-dimensional image formation under single-photon ultra-short pulsed illumination", Proc. SPIE 7378, Scanning Microscopy 2009, 737827 (22 May 2009); https://doi.org/10.1117/12.822773

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