Application of microscopic image dynamic range enhancement in sputum smear tuberculosis intelligent examination

Ping Zhong; Nian Luo; Chen-jie Song

doi:10.1117/12.835601

31 August 2009 Application of microscopic image dynamic range enhancement in sputum smear tuberculosis intelligent examination

Ping Zhong, Nian Luo, Chen-jie Song

Author Affiliations +

Proceedings Volume 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging; 73824F (2009) https://doi.org/10.1117/12.835601
Event: International Symposium on Photoelectronic Detection and Imaging 2009, 2009, Beijing, China

Abstract

The bacteriological microscopic examination of sputum smear for tuberculosis is the most important means of diagnosis and experiments for tuberculosis. Through micro-imaging systems, machine vision systems, digital image processing and computer pattern recognition technology, dynamic intelligent recognition and counting of TB-DNA could be realized. However, the dynamic range of CCD image sensor is limited. The information of TB-DNA could not be fully recorded on microscopic image. In this paper, an effective method to extend the image dynamic range through merging multiple exposure images is proposed. The microscopic images of the same scene with different exposure are taken by rotating the disk-shaped optical grads attenuator that installed under the objective lens of microscopic system to control the illumination. These different exposure images are processed firstly to get irradiance response function of the imaging system, and then the high dynamic range microscopic image (HDRMI) could be obtained. Through the mapping algorithm the dynamic range of HDRMI is compressed in order to been displayed on the general display devices, which not only highlight the feature information of bacillus but also maintain the overall contrast of original microscopic image. The method proposed can effectively express the image information of the bright areas and dark areas in the scenes and enhance the image details and color characteristics. The quality of micro-imaging systems is improved. It is proved that the method proposed in the paper can enhance the resolution and stability of the TB image recognition through the experiment.

Citation Download Citation

Ping Zhong, Nian Luo, and Chen-jie Song "Application of microscopic image dynamic range enhancement in sputum smear tuberculosis intelligent examination", Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 73824F (31 August 2009); https://doi.org/10.1117/12.835601

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available