Biological effects induced by low energy x-rays: effects of nanoparticles

S. Liehn; C. Le Sech; E. Porcel; B. Zielbauer; J. Habib; S. Kazamias; O. Guilbaud; M. Pittman; D. Ros; M.-A. Hervé du Penhoat; A. Touati; H. Remita; S. Lacombe

doi:10.1117/12.832686

28 September 2009 Biological effects induced by low energy x-rays: effects of nanoparticles

S. Liehn, C. Le Sech, E. Porcel, B. Zielbauer, J. Habib, S. Kazamias, O. Guilbaud, M. Pittman, D. Ros, M.-A. Hervé du Penhoat, A. Touati, H. Remita, S. Lacombe

Author Affiliations +

Proceedings Volume 7451, Soft X-Ray Lasers and Applications VIII; 74510Z (2009) https://doi.org/10.1117/12.832686
Event: SPIE Optical Engineering + Applications, 2009, San Diego, California, United States

Abstract

Samples of plasmid DNA were irradiated with pulsed 18.9 nm radiation originating from a Mo X-ray laser (XRL) pumped in GRIP configuration at the LASERIX facility. Up to 21 000 pulses were delivered with a repetition rate of 10 Hz and average pulse energy of 200 nJ. Radiosensitization by two different platinum compounds (platinum terpyridine chloride (PtTC) and platinum nanoparticles) were investigated. SSB and DSB yields were measured using agarose gel electrophoresis. The occurrence of single and double strand breaks not present in controls having undergone the same treatment except for the XRL irradiation can be seen as a clear effect of the XRL irradiation. This confirms the role of direct effects in DNA damages as previously seen with low energy ions and electrons (1) (2). In addition we demonstrate a DNA breaks enhancement in the presence of platinum. No difference of enhancement was seen between these two radiosensitizers.

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S. Liehn, C. Le Sech, E. Porcel, B. Zielbauer, J. Habib, S. Kazamias, O. Guilbaud, M. Pittman, D. Ros, M.-A. Hervé du Penhoat, A. Touati, H. Remita, and S. Lacombe "Biological effects induced by low energy x-rays: effects of nanoparticles", Proc. SPIE 7451, Soft X-Ray Lasers and Applications VIII, 74510Z (28 September 2009); https://doi.org/10.1117/12.832686

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