A comparison study of microarrays by fluorescence imaging and surface plasmon resonance imaging

Zhiyi Liu; Chongwen Zhang; Bin Yang; Xinyuan Chong; Le Liu; Suihua Ma; Yonghong He; Guang Zhang; Jihua Guo

doi:10.1117/12.843435

28 October 2009 A comparison study of microarrays by fluorescence imaging and surface plasmon resonance imaging

Zhiyi Liu, Chongwen Zhang, Bin Yang, Xinyuan Chong, Le Liu, Suihua Ma, Yonghong He, Guang Zhang, Jihua Guo

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 751902 (2009) https://doi.org/10.1117/12.843435
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

In the past decade the analysis of microarrays which are rich in biology information has generated considerable interest. One of the conventional techniques in this area is to detect the biomolecules labeled with fluorescence agents. In recent years, the surface plasmon resonance (SPR) imaging plays an important role in the detection of microarrays. The intensity of fluorescence signal acquired from fluorescence imaging is proportional to the amount of biomolecules, while the refractive index obtained from SPR imaging is relative to the concentration of sample, a comparison study of microarrays by these two detection techniques may be of great value. We develop a quasi-confocal parallel scan fluorescence imaging system which has only one moving part and can produce wide-field confocal images. The bacterial 16s rDNA universal primer labeled with CY5 fluorescence agents are used as probes and prepared as a microarray. The DNA-array is detected by both the quasi-confocal parallel scan fluorescence imaging system and the parallel scan spectral SPR imaging system. The results from these two imaging systems were compared and discussed in resolving power, homogeneity, etc. The refractive index information from the SPR imaging system and the fluorescence intensity information from the fluorescence imaging system are linked by bio-array concentration. The measured results can be inter-referred for bio-array studies.

Citation Download Citation

Zhiyi Liu, Chongwen Zhang, Bin Yang, Xinyuan Chong, Le Liu, Suihua Ma, Yonghong He, Guang Zhang, and Jihua Guo "A comparison study of microarrays by fluorescence imaging and surface plasmon resonance imaging", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 751902 (28 October 2009); https://doi.org/10.1117/12.843435

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KEYWORDS

Luminescence

Imaging systems

Refractive index

Surface plasmons

Charge-coupled devices

Mirrors

Spectral resolution

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