Rotational dynamics and polarization anisotropy of bound and unbound receptor-targeted contrast agents in cancerous and normal prostate tissues studied by time-resolved fluorescence spectroscopy

Y. Pu; W. B. Wang; S. Achilefu; R. R. Alfano

doi:10.1117/12.840775

25 February 2010 Rotational dynamics and polarization anisotropy of bound and unbound receptor-targeted contrast agents in cancerous and normal prostate tissues studied by time-resolved fluorescence spectroscopy

Y. Pu, W. B. Wang, S. Achilefu, R. R. Alfano

Author Affiliations +

Proceedings Volume 7561, Optical Biopsy VII; 75610A (2010) https://doi.org/10.1117/12.840775
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Temporal profiles of polarized fluorescence emitted from receptor-targeted contrast agents: Cybesin and Cytate, in prostate tissues were studied using ultrafast time-resolved spectroscopy. An analytical model was developed and used to investigate rotational dynamics and fluorescence polarization anisotropies of the contrast agents in prostate tissues from the measured data. The differences of rotational times and polarization anisotropies were observed for Cybesin (Cytate) in cancerous and normal prostate tissues, which reflect changes of micro-structures of cancerous and normal tissues, and their different bound affinity with contrast agents. This research may be used to develop better optical methods for in situ prostate cancer detection.

Citation Download Citation

Y. Pu, W. B. Wang, S. Achilefu, and R. R. Alfano "Rotational dynamics and polarization anisotropy of bound and unbound receptor-targeted contrast agents in cancerous and normal prostate tissues studied by time-resolved fluorescence spectroscopy", Proc. SPIE 7561, Optical Biopsy VII, 75610A (25 February 2010); https://doi.org/10.1117/12.840775

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