Laser induced fluorescence lifetime characterization of Bacillus endospore species using time correlated single photon counting analysis with the multi-exponential fit method

Clint Smith; Jarrod Edwards; Andmorgan Fisher

doi:10.1117/12.849219

4 May 2010 Laser induced fluorescence lifetime characterization of Bacillus endospore species using time correlated single photon counting analysis with the multi-exponential fit method

Clint Smith, Jarrod Edwards, Andmorgan Fisher

Author Affiliations +

Proceedings Volume 7687, Active and Passive Signatures; 76870B (2010) https://doi.org/10.1117/12.849219
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

Rapid detection of biological material is critical for determining presence/absence of bacterial endospores within various investigative programs. Even more critical is that if select material tests positive for bacillus endospores then tests should provide data at the species level. Optical detection of microbial endospore formers such as Bacillus sp. can be heavy, cumbersome, and may only identify at the genus level. Data provided from this study will aid in characterization needed by future detection systems for further rapid breakdown analysis to gain insight into a more positive signature collection of Bacillus sp. Literature has shown that fluorescence spectroscopy of endospores could be statistically separated from other vegetative genera, but could not be separated among one another. Results of this study showed endospore species separation is possible using laser-induce fluorescence with lifetime decay analysis for Bacillus endospores. Lifetime decays of B. subtilis, B. megaterium, B. coagulans, and B. anthracis Sterne strain were investigated. Using the Multi-Exponential fit method data showed three distinct lifetimes for each species within the following ranges, 0.2-1.3 ns; 2.5-7.0 ns; 7.5-15.0 ns, when laser induced at 307 nm. The four endospore species were individually separated using principle component analysis (95% CI).

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Clint Smith, Jarrod Edwards, and Andmorgan Fisher "Laser induced fluorescence lifetime characterization of Bacillus endospore species using time correlated single photon counting analysis with the multi-exponential fit method", Proc. SPIE 7687, Active and Passive Signatures, 76870B (4 May 2010); https://doi.org/10.1117/12.849219

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KEYWORDS

Luminescence

Statistical analysis

Single photon

Absorbance

Biological research

Laser induced fluorescence

Signal detection

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