Fused oblique incidence reflectometry and confocal fluorescence microscopy

Matthew D. Risi; Andrew R. Rouse; Arthur F. Gmitro

doi:10.1117/12.875974

22 February 2011 Fused oblique incidence reflectometry and confocal fluorescence microscopy

Matthew D. Risi, Andrew R. Rouse, Arthur F. Gmitro

Proceedings Volume 7893, Endoscopic Microscopy VI; 78930F (2011) https://doi.org/10.1117/12.875974
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure, but relies on exogenous fluorophores, has a relatively limited penetration depth (100 μm) and field of view (700 μm), and produces a high rate of detailed information to the user. A new catheter based multi-modal system has been designed that combines confocal imaging and oblique incidence reflectometry (OIR), which is a non-invasive method capable of rapidly extracting tissue absorption, μa, and reduced scattering, μ's, spectra from tissue. The system builds on previous developments of a custom slit-scan multi-spectral confocal microendoscope and is designed to rapidly switch between diffuse spectroscopy and confocal fluorescence imaging modes of operation. An experimental proof-of-principle catheter has been developed that consists of a fiber bundle for traditional confocal fluorescence imaging and a single OIR source fiber which is manually redirected at +/- 26 degrees. Diffusely scattered light from each orientation of the source fiber is collected via the fiber bundle, with a frame of data representing spectra collected at a range of distances from the OIR source point. Initial results with intralipid phantoms show good agreement to published data over the 550-650 nm spectral range. We successfully imaged and measured the optical properties of rodent cardiac muscle.

Citation Download Citation

Matthew D. Risi, Andrew R. Rouse, and Arthur F. Gmitro "Fused oblique incidence reflectometry and confocal fluorescence microscopy", Proc. SPIE 7893, Endoscopic Microscopy VI, 78930F (22 February 2011); https://doi.org/10.1117/12.875974

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