Combined two-photon microscopy and optical coherence tomography using individually optimized sources

Bosu Jeong; Byunghak Lee; Min Seong Jang; Hyoseok Nam; Hae Koo Kim; Sang June Yoon; Junsang Doh; Sang-Joon Lee; Bo-Gie Yang; Myoung Ho Jang; Ki Hean Kim

doi:10.1117/12.876000

28 February 2011 Combined two-photon microscopy and optical coherence tomography using individually optimized sources

Bosu Jeong, Byunghak Lee, Min Seong Jang, Hyoseok Nam, Hae Koo Kim, Sang June Yoon, Junsang Doh, Sang-Joon Lee, Bo-Gie Yang, Myoung Ho Jang, Ki Hean Kim

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79030Q (2011) https://doi.org/10.1117/12.876000
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Two-photon microscopy (TPM) and optical coherence tomography (OCT) are 3D tissue imaging techniques based on different contrast mechanisms. We developed a combined system of TPM and OCT to provide information of both imaging modalities for in-vivo tissue study. TPM and OCT were implemented by using separate light sources, a Ti-Sapphire laser and a wavelength-swept source centered at 1300 nm respectively, and scanners. Light from the two sources was combined for the simultaneous imaging of tissue samples. TPM provided molecular, cellular information of tissues in the region of a few hundred microns on one side at a sub-cellular resolution, and ran at approximately 40 frames per second. OCT provided structural information in the tissue region larger than TPM images at a sub-tenth micron resolution by using 0.1 numerical aperture. OCT had the field of view of 800 um × 800 um based on a 20x objective, the sensitivity of 97dB, and the imaging speed of 0.8 volumes per second. This combined system was tested with simple microsphere specimens, and then was applied to image the explanted intestine of a mouse model and the plant leaves. Morphology and micro-structures of the intestine villi and immune cells within the villi were shown in the intestine image, and chloroplasts and various microstructures of the maize leaves were visualized in 3D by the combined system.

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Bosu Jeong, Byunghak Lee, Min Seong Jang, Hyoseok Nam, Hae Koo Kim, Sang June Yoon, Junsang Doh, Sang-Joon Lee, Bo-Gie Yang, Myoung Ho Jang, and Ki Hean Kim "Combined two-photon microscopy and optical coherence tomography using individually optimized sources", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030Q (28 February 2011); https://doi.org/10.1117/12.876000

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