Rapid identification of Yersinia pestis and Brucella melitensis by chip-based continuous flow PCR

Michael Dietzsch; Nadine Hlawatsch; Falk Melzer; Herbert Tomaso; Claudia Gärtner; Heinrich Neubauer

doi:10.1117/12.920473

16 May 2012 Rapid identification of Yersinia pestis and Brucella melitensis by chip-based continuous flow PCR

Michael Dietzsch, Nadine Hlawatsch, Falk Melzer, Herbert Tomaso, Claudia Gärtner, Heinrich Neubauer

Proceedings Volume 8367, Smart Biomedical and Physiological Sensor Technology IX; 83670F (2012) https://doi.org/10.1117/12.920473
Event: SPIE Defense, Security, and Sensing, 2012, Baltimore, Maryland, United States

Abstract

To combat the threat of biological agents like Yersinia pestis and Brucella melitensis in bioterroristic scenarios requires fast, easy-to-use and safe identification systems. In this study we describe a system for rapid amplification of specific genetic markers for the identification of Yersinia pestis and Brucella melitensis. Using chip based PCR and continuous flow technology we were able to amplify the targets simultaneously with a 2-step reaction profile within 20 minutes. The subsequent analysis of amplified fragments by standard gel electrophoresis requires another 45 minutes. We were able to detect both pathogens within 75 minutes being much faster than most other nucleic acid amplification technologies.

Citation Download Citation

Michael Dietzsch, Nadine Hlawatsch, Falk Melzer, Herbert Tomaso, Claudia Gärtner, and Heinrich Neubauer "Rapid identification of Yersinia pestis and Brucella melitensis by chip-based continuous flow PCR", Proc. SPIE 8367, Smart Biomedical and Physiological Sensor Technology IX, 83670F (16 May 2012); https://doi.org/10.1117/12.920473

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