The cell-cycle dependence of the spectra of proliferating normal and neoplastic single cells using confocal resonance Raman microspectroscopy

Susie Boydston-White; Cheng-Hui Liu; Robert R. Alfano

doi:10.1117/12.2008738

19 March 2013 The cell-cycle dependence of the spectra of proliferating normal and neoplastic single cells using confocal resonance Raman microspectroscopy

Susie Boydston-White, Cheng-Hui Liu, Robert R. Alfano

Author Affiliations +

Proceedings Volume 8577, Optical Biopsy XI; 85770C (2013) https://doi.org/10.1117/12.2008738
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Confocal resonance Raman (RR) spectra were collected from single proliferating cells and analyzed to detect spectral patterns that are cell-cycle dependent, as a consequence of cellular proliferation — normal or abnormal. The cells’ biochemical age at each time point was confirmed by immunohistochemical staining to identify the presence or absence of cellular components that appear and/or disappear as the cells proceed through the cell-cycle. The RR spectra were collected and compared for each time point as the cells proceeded through the cell cycle to determine what spectral vibrational patterns are cell-cycle dependent. In this study, the question is whether the cell-cycle dependent RR spectral patterns of the vibrational modes observed in proliferating normal and neoplastic single cells are due to a state of cancer or are simply the consequences of the cells’ changing internal biochemistry due to the process of cellular proliferation --- normal or abnormal.

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Susie Boydston-White, Cheng-Hui Liu, and Robert R. Alfano "The cell-cycle dependence of the spectra of proliferating normal and neoplastic single cells using confocal resonance Raman microspectroscopy", Proc. SPIE 8577, Optical Biopsy XI, 85770C (19 March 2013); https://doi.org/10.1117/12.2008738

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